Proje Geliştirme ve
Koordinasyon Birimi

2016-06-22 Egypt: Acute and Chronic Borrelia Infection and Vector Borne Bacterial Co-Infections


Acute and Chronic Borrelia Infection and Vector Borne Bacterial Co-Infections

Description of the project offered: 
Lyme disease, a tick borne disease and co-infections have a worldwide distribution. In Egypt, so far underestimated, it now emerges as a zoonosis[1]. Borrelia spp have been shown in ticks collected in Egypt [2]. In animals, the disease is poorly investigated, but known that tick feed on animals as on humans and animals harbor Borrelia spp, there is a probability to cause a disease under particular immunological circumstances in animals.
A broad range of symptoms [3] are described in humans including, in a chronic stage, arthritis. An comprehensive body of research has been performed during the last decade on human lyme associated arthritis [4] , [5] .

the aim of the project to:

1. Develop a diagnostic test for Borrelia species in animal joint fluid. Since Borrelia species have a regional distribution, the test system would have to consider local Borrelia species. 
2. Develop test systems for direct detection of Borrelia in the blood of animals based on molecular methods and on culturing.
3. Develop tests for T-cell function in infected animals. In humans, the Elispot or similar methods are in clinical use.
4. Develop a characterization by cell frequency of the immunology in lyme and other infections in animals.
5. investigate Joint fluid by cytoflow on the presence of immunocompetent cells.


[1] Elhelw RA1, El-Enbaawy MI1, Samir A2. Lyme borreliosis: A neglected zoonosis in Egypt. Acta Trop. 2014 Dec;140:188-92. doi: 10.1016/j.actatropica.2014.09.005. Epub 2014 Sep 18. 
[2] Adham FK1, El-Samie-Abd EM, Gabre RM, El Hussein H. Detection of tick blood parasites in Egypt using PCR assay II- Borrelia burgdorferi sensu lato. J Egypt Soc Parasitol. 2010 Dec;40(3):553-64.
[3] Borgermans L1, Goderis G2, Vandevoorde J1, Devroey D1. Relevance of chronic lyme disease to family medicine as a complex multidimensional chronic disease construct: a systematic review. Int J Family Med. 2014;2014:138016. doi: 10.1155/2014/138016. Epub 2014 Nov 24.
[4] Hammouda NA1, Hegazy IH, el-Sawy EH. ELISA screening for Lyme disease in children with chronic arthritis. J Egypt Soc Parasitol. 1995 Aug;25(2):525-33.
[5] Baldwin KD1, Brusalis CM1, Nduaguba AM1, Sankar WN2. Predictive Factors for Differentiating Between Septic Arthritis and Lyme Disease of the Knee in Children.
J Bone Joint Surg Am. 2016 May 4;98(9):721-8. doi: 10.2106/JBJS.14.01331.
Project proposer:   Ahmed Elmesiry (Egypt)
Partner role:  Project coordinator 
Partner organisation:  Research
Call for proposal identifier:  H2020-MSCA-IF-2017

Description of the collaboration sought: 

Expertise sought: Scientific research, Veterinary and animal sciences, Medical biotechnology, Life sciences, Biotechnology, Medicine. Health, Industrial manufacture, 
Roles sought: Project coordinator, Project participant, 
Organisation types sought: Consultancy, Research, Education, Public administration, Industry, Technology transfer, Non-governmental organisation (NGO), Small or medium-sized enterprise (SME), , 
Countries sought: Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, United Kingdom, Albania, Bosnia and Herzegovina, Croatia, Iceland, Israel, Liechtenstein, Macedonia, the former Yugoslav Republic of, Montenegro, Norway, Serbia, Switzerland, Turkey, 

Call for proposal specific information
The referenced call for proposals has no specific fields defined.