Proje Geliştirme ve
Koordinasyon Birimi

2016-01-29 Egypt: Embryo Transfer in Sheep and Goat

Embryo Transfer in Sheep and Goat

Description of the project offered: 
Embryo transfer (ET) is a reproductive technology that allows producers to take advantage of high-quality genetics in their dams. In addition, ET can be used to rapidly introduce new genetic lines or entirely new breeds into existing herds.
The standard technique used for the recovery involves retrograde flushing of the uterine horns laparotomy. This method was first described some of ovine embryos and oviducts at sixty years ago. As was commonly found by other researchers.
Repeated laparotomies and handling of the reproductive tract can traumatize tissues and increase the incidence of abdominal adhesions, thereby reducing the potential useful longevity of recipient ewes. Because of laparoscopy’s minor intrusive nature, incision sites are smaller and the incidence of bacterial infection, tissue dehydration and adhesion formation is less than that incurred using laparotomy. 
Laparoscopy is a safe, minor-invasive surgical procedure serving a valuable role in biomedical research. Roberts first reported an ovine laparoscopic technique using a refined fiber-optic system and trocar cannula approach through the ventral abdominal wall. 
Laparoscopy has been effectively used in the ewe to study estrous behavior-pituitary-ovarian relationships, ovarian morphology and ovulation rate. Laparoscopic pregnancy diagnosis, follicular aspiration and more recently, artificial insemination also have been shown to have practical application in sheep. 
Laparoscopic techniques were introduced for embryo recovery to reduce the extent of surgical intrusion. Despite lowering the level of surgical intrusion, laparoscopic techniques, like surgical collection, may lead to adhesions of the reproductive tract and ovaries. This limits the number of times a sheep can be used as a donor. 
Comparison of the laparoscopic and surgical transfer of embryos showed that the laparoscopic method can achieve high pregnancy rates. It appears, that laparoscopic transfer is a safe, minimally invasive surgical procedure and it should be recommended for transfer of embryos in small ruminants. 
In our Institute, the sheep serves a useful role in embryo research and as a model for similar developmental studies in ruminants. Because wildlife species are stress-susceptible, a continuing effort is made to develop or modify manipulative procedures to be as atraumatic as possible. It was hypothesized that laparoscopy might be a safe, efficient method for trans abdominal embryo transfer to a predesignated site within the uterine horn of the recipient. 

The objective of this study was to develop a laparoscopic technique for transferring sheep embryos that may have future application in non-domestic species.
Project proposer:   Ahmed Elmesiry (Egypt)
Partner role:  Project coordinator 
Partner organisation:  Research
Call for proposal title:  N/A 

Description of the collaboration sought: 

Expertise sought: 
Roles sought: Project participant, Project coordinator, 
Organisation types sought: 
Countries sought: Croatia, Iceland, Israel, Liechtenstein, Macedonia, the former Yugoslav Republic of, Montenegro, Norway, Serbia, Switzerland, Turkey, Australia, Brazil, China, Japan, Korea, Democratic People's Republic of, Korea, Republic of, Kuwait, South Africa, United Arab Emirates, United States, Malaysia, Mexico, Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, United Kingdom, Albania, Bosnia and Herzegovina, New Zealand, Swaziland, Thailand, Ukraine